polyclonal guinea pig anti-calbindin  (Synaptic Systems)

Journal: PLOS One

Article Title: Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model

doi: 10.1371/journal.pone.0346246

Figure Lengend Snippet: Number of ChAT + (A-C), GAD-67 + (D), Calbindin + (E), Parvalbumin + (F), Parvalbumin + −Calbindin + (G), Parvalbumin + −GAD-67 + (H), and ChAT + −GAD-67 + (I) cells per biological replicate. The number of cells analysed is the sum of immunostained positive cells from eight spinal cord cross-sections (two sections per lumbar level L3–L6) per biological replicate. Data are shown as mean ± standard deviation and were analysed by an ordinary one-way ANOVA. N = 3 biological replicates per genotype.

Article Snippet: Following three 10-min PBS washes at 100 rpm, secondary probing was performed for 3 hours in the dark in PBS containing 0.05% Tween-20 and the relevant secondary antibodies for ChAT (1:250 dilution, donkey anti-goat IgG H + L Alexa Fluor ® 405, Abcam, ab175664), Calbindin (1:500 dilution, Rhodamine TRITC-AffiniPure donkey anti-guinea pig Ig G H + L, Jackson Immuno Research, 706-025-148-JIR), Parvalbumin (1:500 dilution, Alexa Fluor 647-AffiniPure donkey anti-rabbit IgG H + L, Jackson Immuno Research, 711-605-152), and GAD-67 (1:500 dilution, donkey anti-chicken IgY H + L Highly Cross Adsorbed Alexa Fluor ® 488, Life Technologies, A78948).

Techniques: Standard Deviation

Journal: eLife

Article Title: Non-allometric expansion and enhanced compartmentalization of Purkinje cell dendrites in the human cerebellum

doi: 10.7554/eLife.105013

Figure Lengend Snippet: ( A ) Example masks from a digitally reconstructed PC (gray) and peripherin fiber (blue) on which two unconnected calbindin (+) PC axons (dark and light green) converge to innervate distinct dendritic compartments. ( B ) Example z-projections of two horizontally oriented Poly PCs hosting axons that emerge from the dendrite or an intermediate zone between somatic and dendritic compartments (orange arrowheads).

Article Snippet: Blocking was done with PBS-TX containing 5% normal donkey serum (NDS) and 5% bovine serum albumin (BSA) for 1 hr at RT followed by incubation in guinea pig anti-calbindin primary antibody (1:1000; Synaptic Systems Cat# 214 004, RRID: AB_10550535 ) solution overnight (18–20 hr) at 4°C with 1% NDS in PBS-TX.

Techniques:

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Non-canonical function of ADAM10 in presynaptic plasticity

doi: 10.1007/s00018-024-05327-8

Figure Lengend Snippet: ADAM10 acts via the syt7 pathway. a Syt7 levels in hippocampus of ADAM10 cKO mice are reduced, while the major mossy fiber calcium buffer calbindin and the vesicle marker VAMP are unchanged. n = 3 acute slice preparations of 3 animals (same slices as in Fig. 4). Unpaired, 2-tailed Student’s test. b Synaptic syt7 associates with ADAM10 in a Ca 2+ -independent manner. Endogenous Co-immunoprecipitations from mouse synaptosomes in presence of 200 µM CaCl 2 or 2 mM EGTA. *Unspecific band. Note the different exposure times for the lower blot. syso: synaptosomes; ctr: control. c Heterologous Co-immunoprecipitations from Neuro-2a cells shows syt7-GFP is in one complex with ADAM10 in both calcium (200 µM) and calcium-free (2mM EGTA) conditions. d , e ADAM10 and syt7 co-localize in primary hippocampal cultures. d Representative maximum projections of STED Xtend superresolution images of mature primary hippocampal cultures stained with a CF488A-pre-labelled ADAM10 antibody (green), syt7 (red) and the presynaptic scaffold bassoon (blue) and line scans. e Proximity ligation assay (PLA) for ADAM10 and syt7 in mature hippocampal cultures showing PLA signals at neuronal processes/axons indicating that both proteins are in close proximity (< 40 nm). Example maximum projection of a spinning disc confocal image showing the PLA signal (blue), cell morphology marker actin (phalloidin, green) and the synaptic marker bassoon (red). See also Figure

Article Snippet: Anti-calbindin (guinea pig) , Synaptic Systems , Cat#214004; RRID:AB_10550535.

Techniques: Marker, Control, Staining, Proximity Ligation Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Non-canonical function of ADAM10 in presynaptic plasticity

doi: 10.1007/s00018-024-05327-8

Figure Lengend Snippet:

Article Snippet: Anti-calbindin (guinea pig) , Synaptic Systems , Cat#214004; RRID:AB_10550535.

Techniques: Plasmid Preparation, Recombinant, Blocking Assay, Control, In Situ, Knock-Out, Software, Microscopy

Journal: eLife

Article Title: Endosomal dysfunction contributes to cerebellar deficits in spinocerebellar ataxia type 6

doi: 10.7554/eLife.90510

Figure Lengend Snippet: List of antibodies.

Article Snippet: Calbindin , Anti-calbindin polyclonal (guinea pig) , Synaptic Systems , 214 004 , 1:500 , 2, 3, 6, 9.

Techniques: